Abstract: Sickle cells are known for their significantly shortened lifespan (10-20 days), which is much shorter than the lifespan (~120 days) of the normal red blood cells (RBCs). Similar to normal RBCs, sickle cells are also challenged by repeated hypoxia cycles as well as mechanical fatigue. To examine the impact of these repeated challenges toward the progressive degradation process of RBCs, we have developed in vitro microfluidic assays for testing RBCs in health and disease under cyclic hypoxia loading or cyclic mechanical loading. Both types of fatigue loading are found to cause significant RBC degradation in a cumulative manner. More importantly, our results show that sickle cells on average degrade much faster than normal healthy RBCs. These results provide new insights into the possible mechanisms underlying the significantly shortened lifespan of sickle cells. The developed assays can be used for drug efficacy screening and potentially disease severity testing in a patient-specific manner.
Biographical Sketch: Ming Dao is the Principal Investigator and Director of MIT’s Nanomechanics Laboratory, and a Principal Research Scientist in the Department of Materials Science and Engineering at MIT. His research interests include nanomechanics of advanced materials, cell biomechanics/biophysics of human diseases, and machine learning for engineering and biomedical applications. He has published over 160 papers in peer-reviewed journals, including Science, Nature Materials, Science Advances, Nature Communications, PNAS, etc. He was ranked within the Top 2% Scientists list established by Ioannidis/Stanford University in all four updates published in June 2019 (single year), October 2020 (single year & career), October 2021 (single year & career), and November 2022 (single year & career). He is also ranked as a top 0.5% researcher in both citation and h-index by Exaly.com (March 2023).
He is a Fellow of the American Society of Mechanical Engineers (ASME) and named the 2012 Singapore Research Chair / Professor in Bioengineering and Infectious Disease by MIT. He was a visiting professor with the National Institute of Blood Transfusion, Paris, France (INTS, 2016-2017) and an adjunct professor with Xi’an Jiaotong University, Xi’an, China (2011-2020). Since 2018, he has been a visiting professor at Nanyang Technological University, Singapore. He has also chaired or co-chaired 18 international symposiums/workshops/webinar series.